In 2011, the CBGP opted to set up a metabolomics/mass spectrometry facility. The platform, that is headed by Dr. Stephan Pollmann, was established and fully productive in spring 2012. The metabolomics laboratory contains equipment for the extraction, pre-purification, and derivatization of small molecules, a triple quadrupole mass spectrometer linked to a gas chromatograph (GC-QqQ-MS/MS), and a hybrid mass spectrometer (ESI-Qq-TOF-MS/MS) that can optionally be coupled to an ultra high performance liquid chromatography (UHPLC) or a nanoLC system. With its high mass accuracy and resolution this set-up facilitates advanced metabolite and small molecule detection.
The metabolomics unit offers metabolomics service to the members of the CBGP at cost price. In addition, the expertise and technology of the facility is made available to external groups on the basis of contracted research projects or scientific collaborations. The service covers absolute quantification of target compounds, such as plant hormones, amino acids, carbohydrates, and their derivatives. Moreover, it offers targeted metabolomics approaches. Following data acquisition and processing, fully evaluated data sets are provided. Additional statistical analysis and custom solutions are also available at an extra charge.
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